Analysis of immunophenotype results in 5 patients with acute megakaryoblastic leukemia / 国际检验医学杂志

Objective To analyze the role of various blood cell surface differentiation antigens (CD) in the diagnosis and differen‐tial diagnosis of acute megakaryoblastic leukemia by detecting the immunophenotype in 5 patients with acute megakaryoblastic leu‐kemia .Methods The flow cytometry produced by the BD company and its matching reagents kits were used to detect CD in 5 pa‐tients with acute megakaryoblastic leukemia .Results All patients expressed CD41 and CD42 with the positive rate of 100% ;the lymphoid antigens ware negative in all cases ;CD13 was positive in 4 cases with the positive rate of 80% ;CD33 was positive in 3 ca‐ses with the positive rate of 60% ;CD14 and CD15 were expressed in each 1 case ,the positive rate was 20% .Conclusion The test results show that the CD41 and CD42 have an important value in the diagnosis and differential diagnosis of acute megakaryoblastic leukemia .At the same time ,the characteristic of series non‐specificityin the blood cells of the patients with acute megakaryoblas‐tic leukemia is verified again .

The Inhibitory Effect of OGT Gene Expression on The Level of tau Phosphorylation / 生物化学与生物物理进展

To investigate the effect of OGT expression inhibited by RNAi on the alteration of tau phosphorylation and glycosylation level in HEK293T cells.The siRNAs targeting OGTgene(OGT-siRNA1～3) were designed and chemically synthesized.The OGT-siRNA1～3 were transfected into HEK293T cells via lipofectamine2000.The efficacy of RNA interference was detected by RT-PCR.The fluorescence of GFP/OGT was counted by fluorescence microscopy after pEGFP/OGT and OGT-siRNA1～3 cotransfected to HEK293T cells for 24 h.The level of tau phosphorylation and glycosylation were detected by Western blot after Plasmid pCI/tau441 and the siRNA cotransfected HEK293T cells for 48 h.OGT-siRNA3(100 nmol/L) could effectively downregulate the expression of OGT gene compared with Mock group(80.0% at mRNA level and 51.3% at protein level).The level of phosphorylation at various sites of the tau was significantly upregulated and the glycosylation was downregulated following the inhibition of OGT gene expression.There is an apparent negative correlation between the modification of phosphorylation and glycosylation of tau protein, the deficient in glucose uptaken/metabolism may be an important pathogenesis of AD.